by Staff Writers
Styria, Austria (SPX) Oct 16, 2017
Biofuels obtained from biomass are becoming increasingly important. Apart from biomethane, however, they cannot be produced efficiently, cheaply and sustainably since the current technological complexity and costs are still too high. Partly to blame is cellulose, a polysaccharide and plant constituent which is not water soluble and thus difficult to process.
Typically, biorefineries use a mix of hydrolytically active enzymes which utilize water molecules to breakdown plant material - as happens in natural degradation processes. Recently, oxidative enzymes were discovered which utilize oxygen and work together with hydrolytic enzymes to break down cellulose more efficiently. But how exactly these oxidative enzymes - also known as LPMOs (lytic polysaccharide monooxygenases) - work, was not known. At this stage that the Graz scientists got to work.
Enzymes under the scanning force microscope
For the study currently published in Nature Communications, the hydrolytically active enzyme Trichoderma reesei CBH I, which has been known for a long time, was observed in a first step. The enzyme adsorbs on the surface of a particle, moves along a polysaccharide chain and step by step cleaves off more and more small parts.
In a further step, it has been observed how the behaviour of the enzymes changes when LPMOs are added to the mix. And here the researchers could establish both that the LPMOs generated more binding sites on the surface for the hydrolytically active enzymes, and that the enzyme dynamics on the surface increased considerably.
A picture is worth a thousand words
However, for a reaction taking place on a solid surface such an approach is not feasible. We wanted to observe and document the step before that, that is, the process of cellulose breakdown," says Manuel Eibinger, lead author of the study at the Institute of Biotechnology and Biochemical Engineering.
Bernd Nidetzky, head of the Institute of Biotechnology and Biochemical Engineering at TU Graz: "The saying comes to mind 'a picture is worth a thousand words'. In this study we wanted to document the processes as they occur in time. And this is what we managed to do."
This research topic is anchored in the Field of Expertise "Human and Biotechnology", one of the five strategic research foci at TU Graz.
Berlin (AFP) Oct 9, 2017
Nearly 100 environmental and poverty fighting groups jointly released a letter Tuesday slamming a UN proposal that backs large-scale use of biofuels in commercial planes. Extensive burning of biofuels would vastly expand the production of palm oil, which critics say drives deforestation, higher CO2 emissions and conflicts with indigenous peoples displaced from their land. The UN's Intern ... read more
Graz University of Technology
Bio Fuel Technology and Application News
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